pFA6a-kanMX6-P3nmt1 Sequence

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LOCUS       PFA6A-KANM   5100 BP DS-DNA   CIRCULAR  SYN       09-NOV-1999
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     promoter        3866..5049
                     /note="nmt promoter"
     CDS             470..1327
                     /note="ampR"
     CDS             complement(2676..3482)
                     /note="kanR"
BASE COUNT     1475 A   1101 C   1136 G   1388 T      0 OTHER
ORIGIN      -
        1 TCTATAGTGT CACCTAAATC GTATGTGTAT GATACATAAG GTTATGTATT AATTGTAGCC
       61 GCGTTCTAAC GACAATATGT CCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC
      121 ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT
      181 GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG
      241 GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT
      301 ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA
      361 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
      421 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA
      481 ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA
      541 CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA
      601 CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT
      661 TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC
      721 CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC
      781 ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC
      841 CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA
      901 GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA
      961 ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT
     1021 GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA
     1081 ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC
     1141 GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT
     1201 TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG
     1261 TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA
     1321 GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA
     1381 TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC
     1441 TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC
     1501 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
     1561 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
     1621 CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
     1681 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
     1741 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
     1801 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
     1861 CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG
     1921 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
     1981 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
     2041 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
     2101 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC
     2161 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG
     2221 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT
     2281 ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGGTTAA CCTGGCTTAT
     2341 CGAAATTAAT ACGACTCACT ATAGGGAGAC CGGCAGATCC GCGGCCGCAT AGGCCACTAG
     2401 TGGATCTGAT ATCATCGATG AATTCGAGCT CGTTTAAACT GGATGGCGGC GTTAGTATCG
     2461 AATCGACAGC AGTATAGCGA CCAGCATTCA CATACGATTG ACGCATGATA TTACTTTCTG
     2521 CGCACTTAAC TTCGCATCTG GGCAGATGAT GTCGAGGCGA AAAAAAATAT AAATCACGCT
     2581 AACATTTGAT TAAAATAGAA CAACTACAAT ATAAAAAAAC TATACAAATG ACAAGTTCTT
     2641 GAAAACAAGA ATCTTTTTAT TGTCAGTACT GATTAGAAAA ACTCATCGAG CATCAAATGA
     2701 AACTGCAATT TATTCATATC AGGATTATCA ATACCATATT TTTGAAAAAG CCGTTTCTGT
     2761 AATGAAGGAG AAAACTCACC GAGGCAGTTC CATAGGATGG CAAGATCCTG GTATCGGTCT
     2821 GCGATTCCGA CTCGTCCAAC ATCAATACAA CCTATTAATT TCCCCTCGTC AAAAATAAGG
     2881 TTATCAAGTG AGAAATCACC ATGAGTGACG ACTGAATCCG GTGAGAATGG CAAAAGCTTA
     2941 TGCATTTCTT TCCAGACTTG TTCAACAGGC CAGCCATTAC GCTCGTCATC AAAATCACTC
     3001 GCATCAACCA AACCGTTATT CATTCGTGAT TGCGCCTGAG CGAGACGAAA TACGCGATCG
     3061 CTGTTAAAAG GACAATTACA AACAGGAATC GAATGCAACC GGCGCAGGAA CACTGCCAGC
     3121 GCATCAACAA TATTTTCACC TGAATCAGGA TATTCTTCTA ATACCTGGAA TGCTGTTTTG
     3181 CCGGGGATCG CAGTGGTGAG TAACCATGCA TCATCAGGAG TACGGATAAA ATGCTTGATG
     3241 GTCGGAAGAG GCATAAATTC CGTCAGCCAG TTTAGTCTGA CCATCTCATC TGTAACATCA
     3301 TTGGCAACGC TACCTTTGCC ATGTTTCAGA AACAACTCTG GCGCATCGGG CTTCCCATAC
     3361 AATCGATAGA TTGTCGCACC TGATTGCCCG ACATTATCGC GAGCCCATTT ATACCCATAT
     3421 AAATCAGCAT CCATGTTGGA ATTTAATCGC GGCCTCGAAA CGTGAGTCTT TTCCTTACCC
     3481 ATGGTTGTTT ATGTTCGGAT GTGATGTGAG AACTGTATCC TAGCAAGATT TTAAAAGGAA
     3541 GTATATGAAA GAAGAACCTC AGTGGCAAAT CCTAACCTTT TATATTTCTC TACAGGGGCG
     3601 CGGCGTGGGG ACAATTCAAC GCGTCTGTGA GGGGAGCGTT TCCCTGCTCG CAGGTCTGCA
     3661 GCGAGGAGCC GTAATTTTTG CTTCGCGCCG TGCGGCCATC AAAATGTATG GATGCAAATG
     3721 ATTATACATG GGGATGTATG GGCTAAATGT ACGGGCGACA GTCACATCAT GCCCCTGAGC
     3781 TGCGCACGTC AAGACTGTCA AGGAGGGTAT TCTGGGCCTC CATGTCGCTG GCCGGGTGAC
     3841 CCGGCGGGGA CGAGGCAAGC TAAACAGATC TCGCCATAAA AGACAGAATA AGTCATCAGC
     3901 GGTTGTTTCA TTTCCTATAT TTTTTTTTTA TTTTTTTATT TTTTAATAAG GGAAAATTTA
     3961 ACGTCTAAGG ATACAGAAGA TTGTTAGCAC ATTAAAGTAA TAAAGGCTTA AGTAGTAAGT
     4021 GCCTTAGCAT GTTATTGTAT TTCAAAGGAC ATAATCTAAA ATAATAACAA TATCATTTCT
     4081 CACAAGTTAT TCAATTTTCT TTTTTTTTTC TAATAATATC AAGAATGTAT TATTTGTTTG
     4141 ACATAAGTCA ACTAATTTAT TTAATATGCT GGATTAATCT TGCAGACATG TAAATTAACA
     4201 AGTTTTAGTC AAATAACGTT GAAGTTTCAA TGAACTCAAA TAATTTCTCT TTTTTTTTAT
     4261 ATAACCATAT ATGTCTAATC TGATTTATAT TTTCCGCAGG ATCAACTGAA GTTATGACAT
     4321 TTGGATTGGA TCACTTATAA CCTTGGTCGC CAAATAATAC AAAAATCAGC GTTATAAAAC
     4381 AAAGAAGGTT TTTGTTAAGA AATTAATCCT CTTTCTTGAT AAGAAAGTTG AACCGAAATT
     4441 GCAGATACTG ATATATGAAA ATAATACCCA CAATTTTGGG AATAGCGCAA GCCTCAATTT
     4501 AAACAATAGG TGAGGACACA TGATAATGAC CTCAATGATT GTTAGAAGAA AAGAGCCTCA
     4561 TTACAAAATC GAAAAATGAA TGGTTGGGTA CAAGTTTCCA AAACATGGTA AAGTGGACTT
     4621 TGCGTATGAG ACGTAAATAG AAAAAAACAC TTGTTATATG TTTTCTAGAA TTATTGTTGT
     4681 CTCTTTATGG TTGGATGATG CAAAATAGTA ATTTCGGTTA GTTGCTGTAA AACACCACGA
     4741 GACAAATAGA TATGGATATT TATTAAATCA GGAAAAACGT AACTCTCGGC TACTGGATGG
     4801 TTCAGTCACC CAACGATTAC TGGGGAGAGA AAACAGGGCA AAAGCAAAGC TTAAAGGAAT
     4861 CCGATTGTCA TTCGGCAATG TGCAGCGAAA CTAAAAACCG GATAATGGAC CTGTTAATCG
     4921 AAACATTGAA GATATATAAA GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA
     4981 ATTAATTATT TCAATCTCAT TCTCACTTTC TGACTTATAG TCGCTTTGTT AAATCATGTC
     5041 TTTAATTAAC CCGGGGATCC GTCGACCTGC AGCGTACGAA GCTTCAGCTG GCGGCCGCGT