VectorsS. pombe plasmids consist of a bacterial origin of replication and selectable marker (usually an antibiotic resistance gene), a yeast selectable marker (usually a metabolic marker) and an autonomous replication sequence (ars) which is responsible for high frequency of transformation. There are no single copy centromere plasmids, as there are in budding yeast, because the fission yeast centromere is too big to be easily encompassed on a shuttle vector. Other features may include various promoters and fusion or tagging sequences.
About the vector tableThe vector table is now on a separate page, here.
The accuracy of this list is not guaranteed, and it is not exhaustive, although we try to keep it up to date. More information about vectors, markers, and expression levels is available on our pombe methods page. (For a detailed directory to our site, start with our home page). If you know of plasmids that we are missing, let us know! We've divided pombe plasmids into four categories:
- General purpose vectors
- Expression vectors
- Tagging and fusion vectors (GFP, GST, HA....)
- Special purpose vectors
Those plasmids that have links on their name have a map on-line. These maps are approximate and distances are often by eye. We do not guarantee accuracy, so clone at your own risk! :-). References are provided where possible (see the fission yeast technical references).
Important note: we are not able to function as a plasmid supply house. We will glad provide materials constructed in our lab, but for other materials, we suggest you contact the original publishing author, AddGene, the ATCC, or the Japanese Yeast resource center.